Ausgewählt Publikationen
Optogenetische Enzymkontrolle
Hafki D, Alda J, Pietrus D, Brakmann S.
Modulating Polymerase Activity Through Light-Oxygen-Voltage Domain Insertion.
ChemBioChem 2024, 25, e202400536; DOI: 10.1002/cbic.202400536.
Seifert S, Ehrt C, Lückfeldt L, Lubeck M, Schramm F, Brakmann S.
Optical Control of Transcription: Genetically Encoded Photoswitchable Variants of T7 RNA Polymerase.
ChemBioChem 2019, 20, 2813–2817; DOI: 10.1002/cbic.201900298.
Seifert S, Brakmann S.
LOV Domains in the Design of Photoresponsive Enzymes.
ACS Chemical Biology 2018, 13, 1914–1920; DOI: 10.1021/acschembio.8b00159.
Gerichtete Evolution und Enzym-Engineering
Ibach J, Dietrich L, Koopmans KR, Nöbel N, Skoupi M, Brakmann S.
Identification of a T7 RNA polymerase variant that permits the enzymatic synthesis of fully 2′-O-methyl-modified RNA.
Journal of Biotechnology 2013, 167, 287–295; DOI: 10.1016/j.jbiotec.2013.07.005.
Söte S, Kleine S, Schlicke M, Brakmann S.
Directed evolution of an error-prone T7 DNA polymerase that attenuates viral replication.
ChemBioChem 2011, 12, 1551–1558; DOI: 10.1002/cbic.201000799.
Heyn B, Pogodalla N, Brakmann S.
The double mutation L109M and R448M of HIV-1 reverse transcriptase decreases fidelity of DNA synthesis by promoting mismatch elongation.
Biological Chemistry 2015, 396, 1315–1323; DOI: 10.1515/hsz-2015-0115.
Übersichtsarbeiten und Bücher
Grigalunas M, Brakmann S, Waldmann H.
Chemical Evolution of Natural Product Structure.
Journal of the American Chemical Society 2022, 144, 3314–3329; DOI: 10.1021/jacs.1c11270.
Brakmann S, Schwienhorst A (Hrsg.).
Evolutionary Methods in Biotechnology: Clever Tricks for Directed Evolution.
Wiley-VCH, Weinheim 2004.
Brakmann S, Johnsson K (Hrsg.).
Directed Molecular Evolution of Proteins: Or How to Improve Enzymes for Biocatalysis.
Wiley-VCH, Weinheim 2002.